Journal: bioRxiv

Article Title: Mucin MUC5B levels are reduced in the colonic epithelia of ulcerative colitis patients

doi: 10.1101/2024.02.21.581373

Figure Lengend Snippet: (A) Scoring system to analyze MUC5B proteins in intestinal tissues. Positively stained cells were quantified per field of view under 10X magnification. At least five fields of view were assessed for each tissue and staining patterns were scored as follows: 0 (negative or very low numbers of positive cells, 0-10%), 1 (low, 10-25%), 2 (moderate, 25-50%) or 3 (high, >50%). (B) Immunohistochemical analysis of MUC5B in an inflamed colonic segment from a UC patient. (C) Quantification of MUC5B staining in colons from non-IBD controls (n=12) and in inflamed colons from UC patients (n=28). Error bars represent SEM, * P < 0.05.

Article Snippet: The plates were incubated at 37°C for 20 min to allow the domes to solidify after which 750 μl of human intestinal organoid growth media (Human IntestiCult Organoid Growth Medium, hIOGM, StemCell Technologies, 06010) was added along with 10 μM ROCK inhibitor (Y-27632, Miltenyi, 130-106-138).

Techniques: Staining, Immunohistochemical staining